Fig. 1. Analysis of embryonic liver and heart. Foetal livers from Epor–/– (A) and Epor+/+ (B) embryos at E12.5 were analysed by TUNEL. Apoptotic cells are stained red. (C-E) E12.5 embryos from Epor+/+ mice were analysed for EpoR production in heart. Immunostaining of EpoR was detected in myocardium and endocardium (C,D); tenfold excess of blocking peptide completely blocked anti-EpoR antibody staining (E). (F,G,I,J) Von Willebrand factor (vWF) staining of E12.5 hearts Epor+/+ mice (F,G) and Epor–/– (I,J) mice. Arrowheads indicate vWF positive cells. (H,K) TUNEL assay of Epor+/+ (H) and Epor–/– (K) embryonic hearts show increased apoptosis in the Epor–/– embryo. Arrowhead indicates TUNEL positive cells. Bars, 0.025 mm (A,B,G,H,J,K) or 0.1 mm (C-F,I).
