Fig. 2. Temporal pathway of erythroid commitment during development and expression of the markers used for analysis of EBs (based on that described by Orkin and Zon (Orkin and Zon, 1997). (A) ‘Cellular status’ denotes stages in lineage determination from uncommitted mesoderm to the terminally differentiated erythroid cell that are useful as a working model for analysis. Below each stage are their corresponding expression markers. The sets in brackets serve as endothelial, rather than hematopoietic, markers for hemangioblast cells. (B) Total RNA from day 8 EBs differentiated with 15% serum (+) or serum-substitute SR1 (–) was monitored for expression of the indicated hematopoietic markers by semi-quantitative RT/PCR.
