Fig. 5. Phenotypes associated with 35S::STY1 and 35S::STY2 plants. For simplicity, all illustrations show 35S::STY1 plants. 35S::STY2 plants are identical to 35S::STY1 plants in all aspects shown. (A) Medial view of a stage 13 35S::STY1 gynoecium. Regions with ectopic epidermal style cells are bordered by a white line. (B) Close up of wild-type style cells. (C) 35S::STY1 style cells. Close up of upper section indicated in A. (D) Close up of lower section indicated in A that displays the border between valve cells and ectopic style cells in a 35S::STY1 valve. (E) Poorly developed anthers (arrowheads) and narrow petals of a 35S::STY1 flower. (F) Lateral view of a 35S::STY1 silique. (G) Inner valve epidermal cells. (H) Transverse section of a wild-type valve. (I) Transverse section of a 35S::STY1 valve. Double headed arrow indicates the region of ectopic style cell formation. A mass of overproliferating chlorenchyma cells is seen above the area with ectopic style cells. (J) 35S::STY1 gynoecium with a dehiscing valve still attached to the style along the area where no dehiscence zone is formed (arrow). (K-N; P-R) Transmission electron micrographs of transverse sections of wild-type and 35S::STY1 valves. (K) Wild-type valve. (L) 35S::STY1 valve. (M) Close up of outer epidermal cells of a 35S::STY1 valve. (N) Close up of inner epidermal cells of a 35S::STY1 valve. (O) Medial view of a 35S::STY1 gynoecia with discontinuous lateral xylem strands (arrowheads) and massive medial xylem fans. (P) Close up of wild-type outer epidermal valve cells. (Q) Close up of wild-type style cells. (R) Close up of 35S::STY1 style cells. (S) Medial view of a 35S::STY1 gynoecia with more severe defects than those in (O). Scale bars: 100 µm in A,E,F,J,O,S; 20 µm in B,C,D. sy, style; e sy, ectopic style; r, replum; v, valve; o e, outer epidermis; i e, inner epidermis.

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