Fig. 2. Temporal expression of immune-related transcripts visualized through RT-PCR analysis. Results for nine transcripts over 10 developmental stages are shown. Stages include four embryonic stages (in hours after fertilization), two larval stages (pre-competent larvae two hours after hatching and competent larvae 10 hours after hatching), and four juvenile stages (time after settlement). The 16S mtl rRNA bands at the bottom were used as a loading control. Transcripts are grouped according to the screens by which they were isolated. The title of each screen is labeled above the relevant set of transcripts and the timing of the subtractive hybridization is indicated by an arrow.

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