Fig. 2. Gli1^lz/lz;Gli2^zfd/+ embryos have defects similar to Gli1^zfd/zfd;Gli2^zfd/+ embryos. In E10.5 Gli1^lz/lz;Gli2^zfd/+ embryos, there is a variable loss of floor-plate cells. In the most extreme mutant embryos, HNF3ß- and Nkx2-2-expressing cells are greatly reduced (compare E and F with A and B), such that Isl1/2-expressing cells occupy the ventral midline of the spinal cord (compare G and H with C and D). (C,G) Sections at forelimb level; (D,H) sections at hindlimb level. Arrowhead indicates ventral midline of spinal cord. (I) At E12.5, Gli1^lz/lz;Gli2^zfd/+ lungs are smaller than wild-type lungs, in addition, Gli1^lz/lz;Gli2^zfd/zfd lungs have only two lobes and are much smaller than wild-type lungs. At E18.5, a postaxial nubbin was found in the limbs of Gli1^lz/lz;Gli2^zfd/zfd embryos (K), similar to Gli1^zfd/zfd;Gli2^zfd/zfd embryos. (J) E18.5 wild-type limb. Scale bar: 0.1 mm in A-H.

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