Fig. 3. Nrp2 expression in adult vessels. (A-C) Transverse sections of P30 gut from a Nrp2^+/– mouse stained with antibodies directed against the indicated proteins. Note co-expression of NRP2 (A) with the lymphatic markers podoplanin (B) and VEGFR3 (C) in subserosal lymphatic vessels (asterisks). Weak NRP2 expression is observed in a subserosal vein (v, arrow). NRP2 is also expressed in the smooth-muscle layer surrounding the gut (Chen et al., 1997), punctuate staining reflects loss of one wild-type allele. (D,E) Staining of Nrp2^+/+ P0 mesentery sections with the indicated antibodies. Note co-expression of NRP2 and VEGFR3 in lymphatics (asterisks), weaker expression of NRP2 in veins (V, arrows), and absence of expression in arteries (A). (F,G) P0 skin section from a Nrp2^+/– mouse double-stained with X-gal (blue staining) and VEGFR3 (F) or podoplanin (G) antibodies (brown staining). Note double-stained lymphatics (asterisks). NRP2 also labels hair-follicles (arrowheads). Scale bars: in A, 140 µm for A-C; in D, 35 µm for D,E; in F, 40 µm for F,G.

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