Fig. 1. Numb expression in the E10.5 mouse cortex in vivo and in acutely isolated cells. (A) Numb staining is seen throughout the E10.5 mouse cerebral cortex. Some ventricular zone cells show strong staining at their apical border. LV, lateral ventricle. (B-H) E10.5 cortical cells were isolated, fixed acutely, stained with anti-Numb antibody and double labeled with an antibody to Nestin or ß-tubulin III. (B) Quantification of Numb⁺ cells in the progenitor cell population (Nestin⁺) and immature neuronal population (ß-tubulin III⁺). (C,F) Phase-contrast images of the fields stained for Numb (D and G, in red), Nestin (E, in green), or ß-tubulin III (H, in green). Arrows indicate cells double labeled for Numb and Nestin (C-E) and Numb and ß-tubulin III (F-H). Scale bars: 100 µm.

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