Fig. 3. Derrière is a direct target of VegT. VegT-GR, a hormone-inducible form of VegT, was injected into Xenopus embryos at the one-cell stage and the embryos were allowed to develop to the late blastula stage. Animal caps were dissected from these embryos and they were incubated in dexamethasone (DEX), cycloheximide (CHX) or both. After 3 hours of culture they were analysed for expression of derrière or Bix4 by RNAase protection. Ornithine decarboxylase (ODC) was used as a loading control. Note that both derrière and Bix4 are induced by DEX in the presence of CHX, but that CHX causes a decrease in levels of activation of derrière.

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