Fig. 5. The derrière 5' regulatory region drives mesendodermal expression of a GFP reporter gene. (A) The reporter construct. (B-D) Views of transgenic Xenopus embryos showing expression of GFP by in situ hybridisation. (B) Stage 10.5 embryo, vegetal view. Expression of GFP is detectable in the mesoderm and (weakly) in the endoderm. (C) Dorsal view of the embryo shown in B. (D) Lateral view of a stage 10.5 embryo with dorsal blastopore lip to the right. This embryo was bisected before the staining procedure. Note that expression of the reporter construct differs from that of the endogenous gene in that transcripts persist in the involuted mesoderm.

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