Fig. 2. Multilineage repopulation with 12 d.p.c. embryonic tissues. The analysis of reconstitution of mice transplanted with embryonic tissues was performed 4 months or longer after transplantation either by FACS analysis (A) or using Y-specific PCR as described previously (B) (Medvinsky and Dzierzak, 1996). (A) Multilineage reconstitution with 12 d.p.c. cells of embryonic tissues was confirmed in some recipients by co-staining for donor Ly5.1 and lineage-specific B220, Mac-1 and CD3 markers. In some reconstituted mice the percentage of Ly5.1+, Mac- 1+ cells was very low (data not shown). (B) Y-specific PCR analysis of blood samples. Each line on the gel represents an individual mouse. The level of reconstitution was assessed with reference to results of PCR using standard dilutions of male DNA in female DNA. Arrowheads point to the mice reconstituted at the level of close to 10% or more.

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