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Fig. 1. Structure of the different P elements used to induce the swapping of
Fab-7 by scs. The 3.1 kb fragment (containing the
Fab-72 deletion) that serves as ectopic donor in the gene
conversion experiment is drawn in dark blue at the scale indicated at the
bottom of the figure. The iab-7PRE abutting the Fab-7
boundary is indicated. This Fab-7 fragment is inserted in front of a
miniwhite gene within a P-element (the feet of the P element are
indicated by black rectangles; the miniwhite gene in red is not drawn at
scale). The structure of the SCS element that was inserted in the
NsiI site just upstream from the Fab72 deletion
endpoint is shown in green above the Fab-7 DNA line with a few
relevant restriction sites (drawn at the same scale; B, BamH1; H,
HpaI: M, MluI; P, PstI). The promoter driving
transcription under the control of the iab cis- regulatory domains
from within the BX-C is indicated in dark green. The promoter described by
Avramova and Thikonov (Avramova and Thikonov, 1999) is shown in light green.
The percentage of pairing sensitive lines from each construct is indicated
with the numbers of lines scored (only homozygous viable lines are
reported).