Fig. 3. RNA in situ hybridization to Uab embryos. (A) An embryo from Uab^HH1/MKRS parents, hybridized with a probe from fragment B to detect distally oriented transcripts. The embryo is at stage 11 (6 hours old), and is dissected to show the whole epidermis in one plane. The anterior end is at the top; the anterior limit of RNA expression is in PS6, as marked. (B) Same as A, except the embryo is stage 13 (10 hours old). (C) Same as A, except that the probe is from fragment I, again detecting distally oriented transcripts. The weak signals represent rare transcripts that have extended 50 kb from the Uab^HH1 insertion. (D) Same as A, except that the embryo was from Uab^R1/MKRS parents. There is less readthrough transcription than in Uab^HH1, presumably because of the intact poly(A) addition signals in the Uab^R1 insertion. Wild-type embryos show no distally directed RNA with probes from either the B or the I fragments.

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