Fig. 2. Deletion analysis of the eve 2 MSE. The lacZ reporter construct used in these assays is shown schematically at the top, and contains the eve 2 MSE and the eve 3+7 MSE to control for levels of expression. Whole-mount lacZ mRNA expression patterns directed by this construct are shown for the wild-type eve 2 MSE (A,B), and for eve 2 MSEs containing deletions (D1-4; C-J) as shown in Fig. 1. Unless otherwise indicated, all embryos in this paper are oriented with anterior towards the left and dorsal upwards. Expression patterns are shown for embryos early (left column) or mid-way through (right column) cleavage cycle 14. The wild-type eve 2 enhancer is first activated in a broad anterior domain (marked by the broad line, A), which is then refined to a stripe (B). Deletions 1, 3 and 4 each cause a failure to activate or maintain wild-type expression levels of eve 2 (C,D,G-J). D2 causes a premature strengthening of eve 2 with a more extensive anterior expansion (E). This expansion refines to form an ectopic stripe (^*) in mid-cycle 14 (F).

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