Fig. 6. Expression of SMA-3::GFP C-terminal fusion protein in different genetic backgrounds. (A-F) sma-3::gfp C-terminal fusion was co-injected with sma-3 genomic fragment, which contains the whole sma-3 gene sequence. (A,B) sma-3(wk30) mutant background. (C,D) sma-4(e729) mutant background. (E,F) sma-6(wk7) mutant background. (G,H) sma-3::gfp C-terminal fusion was co-injected with sma-3 genomic lacking coding sequences. All of the photos are taken with the same exposure time. The prominent fluorescence in B is autofluorescence of the intestine. (I) Western blot of total protein extracts from strains carrying sma-3::gfp(C) constructs. The extra-chromosomal arrays were integrated into N2 (qcIs12[sma-3::gfp(C) + sma-3 + rol-6] and qcIs16[sma-3::gfp(C) + sma-3no-code + rol-6]) and crossed into different genetic backgrounds. Equal amounts of total protein were loaded in each lane and SMA-3::GFP fusion protein was detected by western blot with anti-GFP antibody.

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