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Fig. 4. Tissue-specific expression of mec-8(+) transgenes in embryos. (A) A 2.5-fold mec-8(u314) embryo carrying Phlh-1::mec-8(+) on mnEx113 stained with anti-MEC-8. (B) Embryo shown in A stained with MH2; UNC-52 localizes in stripes over muscle cell quadrants at this stage. (C) Merged images of A and B. (D) mec-8(u314) embryo carrying Pdpy-7::mec-8(+) on mnEx134 stained with anti-MEC-8. (E) Embryo shown in D stained with DM5.6; myosin is diffuse and outlines muscle cell nuclei (arrows) at this stage. (F) Merged images of D and E. (G) 7 second exposure of an embryo carrying muscle-specific unc-52(e444)::gfp minigene mnIs25. (H) 7 second exposure of a mec-8; unc-36; mnIs25; mnEx113 embryo. Scale bar: 10 µm.





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