Fig. 4. Genomic structure of C. elegans wee-1.3 and location of mutations. (A) The eight exons (including the trans spliced SL1 leader exon) of wee-1.3 RNA are indicated by differently shaded or filled rectangles. Introns are the lines between exons. The spe-37(gf) mutations are listed within a large box above exon 7. The missense suppressor mutations (eb94, eb62, eb90 and eb88) and the associated amino acid changes are indicated above exon 3. The two suppressor mutations that cause a premature stop (eb93 and eb60) are indicated below exon 7. The eb91 deletion that removes exon 2 and part of exon 3 (including the intron acceptor for trans spliced exon 1) is indicated below these exons. The eb61 suppressor mutation alters the intron 4 splice donor (shown in the box above intron 4), while the eb87 suppressor alters the intron 4 splice acceptor site mutation (shown in the box below intron 4). (B) Comparison of wee-1.3 mRNA produced by wild-type (top) and spe-37(q89 eb87) suppressor mutant homozygotes (bottom). Splicing is altered so that the exon 5 sequence encoding the predicted transmembrane domain is absent.

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