Fig. 5. Alignment of C. elegans, C. briggsae, Xenopus, mouse and human wee-1.3 kinases. Red boxes indicate identical residues among all wee-1.3 orthologs. Black boxes indicate identity to the C. elegans sequence. Asterisks indicate the sites of mutation in C. elegans spe-37(gf), missense and nonsense suppressors. The phenotypic class of each missense or nonsense suppressor is indicated in parentheses (see Table 1). Alignment was performed using the ClustalX program (Thompson et al., 1997). The C. briggsae wee-1.3 sequence is publicly available unpublished data from the C. briggsae Genome Project (The Sanger Institute Cambridge, UK and The Genome Sequencing Center, Washington University, St Louis, MO). C. briggsae wee-1.3 cDNA was predicted using GeneMark hmm (Borodovsky, 1998) and sequence around the spe-37(gf) region was verified by examination of ABI sequence traces (see http://trace.ensembl.org). ATP, ATP-binding domain; kinase, Wee1p-like kinase domain; TM, predicted transmembrane domain. The GenBank Accession Number for C. elegans wee-1.3 is NP_496095, C. briggsae wee-1.3 is based on the GeneMark HMM prediction from contig C000100543, Xenopus Myt1 is A57247, Mouse Myt1 is NP_075545 and Human Myt1 is NP_004194.

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