Fig. 8. In situ hybridization for Sema3c (A-C) and Sema5a (D-G) in wild-type (A,D,E), heterozygous (B) and Pax6^-/- (C,F,G) forebrains at E14.5. (A,B) In wild-type and heterozygous brains, Sema3c expression is seen within the subventricular (svz) and intermediate zone (iz) of the entire pallium, with a most prominent signal detected within svz/iz of the lateral and ventral pallium (arrows). The signal had sharp ending at the PSPB. (C) In the Pax6^-/- forebrain, the prominent expression of Sema3c in the svz/iz of the ventrolateral pallium is not detectable, while the expression in the dorsal pallium is shifted to the outermost surface of the developing cortical plate. (D-G) The normal strong expression of Sema5a in the ventricular zone (vz) of the entire pallium (D,E) is substantially diminished in the neuroepithelium of the Pax6^-/- brains (F,G). The arrowhead in G indicates to the strongly affected expression of Sema5a in the vz of the ventrolateral pallium of the Pax6^-/- brains, when compared with the expression in wild-type (arrow in E). GE, ganglionic eminences; se, septal eminence. Scale bars: 200 µm in A-C,G,I; 100 µm in D-F,H,J.

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