Fig. 3. CSN5 is required for dorsoventral patterning of the embryo. (A) In wild-type embryos dpp RNA localizes to the dorsal side of the egg as well as the two poles. (B) In eggs from CSN5 GLC mothers, the dpp-expressing domain is often reduced, indicating that the embryo is ventralized. (C) In rare, dorsalized embryos, dpp RNA extends around the DV circumference. (D) In wild-type embryos, twi RNA is expressed in the ventral mesoderm and at both poles. (E,F) twi RNA in embryos derived from CSN5 germline mothers. (E) In ventralized embryos, twi RNA is more broadly expressed ventrally and laterally. (F) In rare, strongly dorsalized embryos, twi RNA fails to accumulate in the mutant egg. (G) In wild-type embryos, rho RNA is expressed in ventrolateral stripes and a dorsal midline stripe. (H) In weakly ventralized eggs derived from CSN5 GLC mothers, the lateral stripe of rho RNA was moved dorsally and the dorsal stripe was reduced. (I) In dorsalized embryos rho RNA accumulates in stripes that are probably derived from the dorsal stripe seen in wild-type embryos. Zygotic expression patterns could not be analyzed in the substantial fraction of embryos that were unfertilized or extremely fragile (see Table 1).

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