Click on image to view larger version.

Fig. 5. The expression of flh and hrT in flh mutant
embryos. (A-D) Lateral views of 17-somite embryos with anterior towards the
top, hybridized with a mixture of flh and hrT probes. In
wild-type embryos or flh heterozygotes (A), flh is expressed
in the tip of the tail (red arrow) and epiphysis (red arrowhead), and
hrT is expressed in the dorsal aorta (black arrowhead), ventral
region of the gut tube (asterisk) and in the heart (black arrow). In
flh homozygotes (B), flh expression is absent and
hrT expression is missing in the dorsal aorta. The gut tube and
cardiac expression of hrT is not affected. (C,D) Higher-power views
of the embryos in A,B, respectively. (C) The dorsal aorta expression of
hrT (black arrowhead) in a wild-type or flh heterozygote.
(D) The complete absence of hrT expression in the dorsal aorta of a
homozygous flh embryo.