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Fig. 3. Effect of FGF treatment on Hoxb4 and Hoxb9 expression in
the neural tube. Dorsal views of stage 14-15 embryos hybridized with
Hoxb4 (A-C) or Hoxb9 (D-F). Untreated embryos (A,D), embryos
treated overnight with FGF2 in culture (B,E), embryos electroporated with an
e-FGF-expressing construct unilaterally in the left side of the neural tube
(C,F). In both cases, Hoxb9 is upregulated by exogenous FGF (E,F)
while Hoxb4 shows no change (B,C). In E, note that the anterior limit
of the Hoxb9 expression reaches the level just posterior to the otic
vesicle. Black arrowheads in F show the extended domain of Hoxb9 on
the left and the control limit on the right in the neural tube.
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