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Fig. 2. Polar cells are required to specify border cells. fng mosaic follicles stained with Hoechst (DNA, white) and antibodies recognizing {pi}-MYC (fng+ cells, green), ß-galactosidase (slbo-lacZ, border cells, red) and FasIII (polar cells, cyan). In all figures, panels marked ' or '' show separate channels of the same follicle. (A) Stage 9 follicle with no fng+ anterior cells. No anterior polar cells are formed and no border cells are specified. Arrowhead indicates posterior polar cells. (B) Stage 10A follicle with only the two polar cells fng+ at the anterior. The polar and outer border cells are specified and properly migrate, even though all outer border cells are fng- (insets).





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