Fig. 3. Generation of dek1 genetic mosaics and examples of an autonomous phenotype. (A) Diagram depicting the strategy for generating plants genetically mosaic for the dek1-792 allele. (B) Fluorescence micrograph of a leaf with mutant epidermal cells over wild-type internal cells. The normal mesophyll cannot rescue the mutant phenotype in the epidermis. The sector caused the leaf to bend, highlighting the influence of the epidermis on leaf morphology. (C) Another fluorescence micrograph of a sector. In the mesophyll, lack of red-fluorescing chloroplasts indicates that the tissue is genetically mutant for dek1. The guard cells are the only epidermal cells to contain chloroplasts. (D) Enlargement of the boxed region shown in C. The red-fluorescing wild-type guard cell (arrowhead) is directly adjacent to an epidermal cell showing a clear mutant phenotype (arrow). (E) Mutant mesophyll cells in a section of leaf with wild-type epidermis. The cells show unusual lobing. (F) Section where the internal mesophyll and bundle sheath cells are wild type (arrows) and the outer mesophyll layer is mutant and shows a mutant phenotype. The epidermis is wild type except for the bulged region between the arrowheads on the adaxial (upper) surface. (G) A single epidermal cell with mutant phenotype (asterisk) surrounded on 3 sides by normal looking cells. The phenotype appears cell-autonomous. (H) A cell with wild-type phenotype (asterisk) surrounded on 3 sides by cells with mutant phenotypes. The arrowhead designates the boundary between abnormal and normal bulliform cells. (I) An example of a cupped hair. Scale bars 100 µm (B-D,F,I); 50 µm (E); 10 µm (G,H).

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