Fig. 6. Wild-type and its intracellular mutants of Xror2 affect convergent extension of neural tissue and notochord but not cell differentiation markers. Embryos were injected with mRNA as indicated, and were subjected at stage 18 to ß-gal staining (red) and whole-mount in situ hybridization with pan-neural marker nrp1 (A-C) or notochord marker XPA26 (D-F) as probes. Right panel, sections of stained embryos; inset, higher magnification of neural tissue or notochord region. Expression domains of nrp1 were widened laterally and shortened anteriorly in Xror2- (B) and Xror2-TM- (C) expressing embryos, compared with globin-expressing embryos (A). nrp1 expression was not inhibited in nß-gal-positive regions of Xror2- (B) and Xror2-TM- (C) expressing embryos (right panel, inset). Expression domains of XPA26 failed to elongate anteriorly and were located near the blastopore in Xror2- (E) and Xror2-TM- (F) expressing embryos, compared with those expressing globin (D) (left panel). Areas of XPA26 expression domains on section are much larger in Xror2- (E) and Xror2-TM- (F) expressing embryos than in globin-expressing embryos (D) (right panel, inset), whereas there is no inhibition of XPA26 expression in nß-gal-positive regions. a, archenteron; arrowhead, collapsed archenteron; arrow, thickened pigmented epithelial layers of the ectoderm.

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