Fig. 4. Repression of Cash1 by Lmx1b and Wnt1 misexpression. (A) Lmx1b misexpression represses Cash1 expression at 48 hours after electroporation. The right-hand side is the experimental side. Rostral is towards the top. Black arrows indicate dorsal midline. By 48 hours after electroporation, some regulation may have occurred, and repression sites are patchy (red arrows). (B) Wnt1 misexpression represses Cash1 expression at 24 hours after electroporation. Both panels are dorsal views of the mesencephalic region of embryos. (A) Flat mount; (B) dorsal view. (C,D) Higher magnification of the areas indicated in B. At 24 hours after electroporation, Cash1 expression is repressed uniformly by Wnt1. Scale bars: 200 µm in A,B; 100 µm in C,D.

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