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Fig. 3. Expression of AP markers in the inner ear. Lateral or dorsolateral views of
the otic vesicle (anterior towards the left). (A-D) pax5 expression
at 24 h (A,B) and 30 h (C,D). Staining is limited to the anterior end of the
otic vesicle in wild-type embryos (A,C) but is greatly expanded in
val/val mutants (B,D). The midbrain-hindbrain border (mhb) is
indicated. (E,F) Expression of nkx5.1 at 24 h in wild-type (E) and
val/val (F) embryos. Expression is expanded posteriorly in
val/val mutants. (G,H) Expression of zp23 at 24 h in
wild-type (G) and val/val (H) embryos. No expression is detectable in
the ear in val/val mutants. Relative positions of rhombomeres are
indicated. Scale bar: 25 µm in A,B,G,H; 75 µm in C,D; 50 µm in
E,F.
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