Fig. 6. Effects of mis-expressing val. (A,B) Dorsal views showing expression of fgf3 (blue) and krox20 (red) at 14 h in a normal embryo (A) and an embryo injected with val RNA (B). The val-injected embryo shows little or no fgf3 expression in the r4 domain (arrowheads) and has undergone less convergence than normal. (C,D) Lateral view of a val-injected embryo at 24 h. Trunk and tail tissues are ablated (C) and no otic vesicle is visible (D). (E,F) Dorsal views of val-injected embryos with relatively normal axial development. (E) Expression of fgf3 (blue) and krox20 (red) at 14 h. The left side of r4 shows little fgf3 expression (arrowhead) whereas the right side is nearly normal (bracket). (F) Expression of pax2.1 at 24 h in the midbrain-hindbrain border (mhb) and otic vesicles (ov). The left otic vesicle (broken circle) is severely disrupted. (G,H) Expression of fgf8 at 12 h in a normal wild-type embryo (G) and a val-injected embryo (H). The val-injected embryo has a truncated axis (not shown) and has undergone less convergence than normal. Nevertheless, fgf8 is expressed relatively normally in the prechordal plate (p), midbrain-hindbrain border (mhb) and rhombomere 4 (r4). Anterior is towards the left in all panels. Scale bar: 100 µm in A,B,D-H; 250 µm in C.

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