Fig. 8. Analysis of Fgf10 expression in Gas1^-/- limbs. Fgf10 expression was detected by ³⁵S-ISH in control (con; A,B,D,F) and Gas1^-/- (-/-; A',B',D',F') forelimb buds at E9.5, E10.0, E10.5 and E11.5 in horizontal sections (n=4). Arrowheads indicate the distal tip mesenchyme underneath the AER; brackets, the region of distal tip mesenchyme with reduced Fgf10 expression in the mutant; white arrowhead, moderately recovered Fgf10 expression at the distal region at E11.5 Fgf10 expression was also assessed by whole-mount ISH in control (C,E) and Gas1^-/- (C',E') forelimbs at E10.0 and E10.5 (dorsal and dorsal-lateral views, respectively). Only 2 out of 12 E10.0 and 2 out of 13 E10.5 embryos showed clear defects of Fgf10 expression in one of the limbs by whole mount.

Return to article