Fig. 2. HoxB7/Cre reporter analysis. HoxB7/Cre mice were crossed to either Rosa (A-F) or Z/AP (G-I) reporter mice and subjected to histological staining to visualize ß-galactosidase activity (A-F; G-I, red, lacZ) or alkaline phosphatase (I, blue, AP) activity. Ages of embryos are indicated. (A-D,G-I) Whole-mount views; (E-F) sections. (A) Mesonephric duct (arrow). (B) Mesonephric duct and onset of ureteric bud invasion into the metanephric mesenchyme (arrow; low levels of HoxB7/Cre activity were also detected in the dorsal root ganglia (arrowhead) and the spinal cord). (C) The ureteric bud has branched once in the metanephric mesenchyme (arrow). (D) The ureteric bud has undergone several branches, the ureter (arrow) and the Wolffian duct (arrowhead) are evident. (E,F) Sections of different regions of a E12.5 kidney. (G-I) The ß-galactosidase activity was high in ureteric bud derivatives of the Z/AP kidney in the absence of HoxB7/Cre (G), but was completely removed in the presence of HoxB7/Cre (H). Alkaline phosphatase activity was detected in all ureteric bud branches (I).

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