Fig. 2. Excess proliferation in axin^- mutant clones. (A) axin^- mitotic clones were induced 60 hours after egg laying (AEL) using the GAL4/GAL80 system and eye/antennal discs were analysed by FACS at 108 hours AEL. Using this marker system, the mutant cells are positively marked by GFP. The black trace represents GFP-negative (wild-type) cells and red line represents GFP-positive (axin^-) cells. The proportion of cells in G2 and S is increased in axin^- cells, consistent with the hyper-proliferation phenotype observed in these clones. (B) An axin^- Minute clone (marked by absence of red) stained with anti-BrdU (green). The focal plane shown is at the level of the clonal overgrowth so the S-phase associated with the second mitotic wave (just posterior to the morphogenetic furrow, arrowhead) is not seen.

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