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Fig. 4. Flat-mount in situ hybridization of E9 chick retinas electroporated with
EnRaxL
C viral construct. The signals of endogenous Brn3a
(A-C), Chx10 (D-F) and Pax6 (G-I) transcripts are shown in
purple and EnRaxL
C viral transcript is shown in green-blue. The
sections of the retina samples in B,E,H are shown in C,F,I, respectively.
Scale bars: in A is 2 mm for A,B,D,E,G,H; in F, 30 µm for C,F,I. GCL,
ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer;
ONL, outer nuclear layer.