Fig. 5. Dendrite defects in late pupal or adult type I sensory neurons. All neurons are labeled with mCD8-GFP. (A) A scheme representing the neuron innervation of a campaniform sensillum of the adult wing vein. Arrowhead indicates the dome-shaped structure of the sensillum. (B-E) Dendrite observation in adult wing campaniform sensilla. Arrowheads indicate the position of the dome shaped sensilla. In control (not shown) or rescued Rfx²⁵³/Rfx⁴⁹ mutant wings (B), the cilium is located just under the dome-shaped sensory structure produced by the accessory cell. In all Rfx²⁵³/Rfx⁴⁹ mutant wings (C-E), the dendrite is never situated under the dome-shaped sensilla and sometimes ends well before the sensillum (C). In most cases, the cilium is either shorter or absent (D,E). (F) A schematic representing the two neurons innervating a chordotonal organ of the femur as observed in G-L. In Rfx²⁵³/TM6B (G) or rescued Rfx²⁵³/Rfx⁴⁹ mutant pupae (H,I), the three subregions of the cilium are clearly visible. tb, tubular bundle; cd, ciliary dilation; ci, cilium; tz, transition zone. In mutant legs (J,L), the cilium is either absent or shorter (arrows) and distinct subregions cannot be distinguished. The dendritic transition zone appears swollen (arrowheads). (J) Rfx⁴⁹/S143702; (K) Rfx⁴⁹/Rfx²⁵³; (L) Rfx²⁵³/S143702.

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