Fig. 6. Functional analysis of clones 56-G6/HMG-X and 57-G10/TAB3 in animal cap explants. (A) Clones 56-G6/HMG-X and 57-G10/TAB3 were injected into animal caps and analyzed for fate changes at stage 20. Both clones induce neural or cement gland marker expression. Notice no induction of posterior neural markers (En-2, mid/hindbrain; Krox-20, hindbrain; twist, neural crest) and weak induction of the heart marker xNkx2.5. (B) 56-G6/HMG-X synergizes with low amounts of Smad7 (5 pg). Notice inhibition of cement gland fates induced by Smad7 in the presence of 56-G6/HMG-X (lane 5). (C-H) Gain-of-function experiments with 56-G6/HMG-X in vivo. Embryos were injected into 1/2 cells in the animal pole and embryos analyzed at tadpole stages. Notice enlargement of the anterior neural plate (C-E), and ectopic expression of the anterior neural marker Pax6 (F-H). Asterisks indicate enlarged anterior neural territory (D,E) and ectopic Pax6 expression (G,H).

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