Fig. 7. The amount of NICD is higher in hephaestus clones relative to normal surrounding tissue. (A,C) Normal NICD staining in a heterozygous heph⁰³⁴²⁹ wing disc. The position of the DV boundary is noted in A (arrowhead). (B-B'',D-D'') A typical example of a heph⁰³⁴²⁹ mosaic wing disc with elevated levels of NICD staining corresponding to the positions of heph mutant clones that are marked by loss of a GFP reporter transgene. (C,D-D'') Higher magnification view of the discs in A,B-B'', respectively. The positions of the XZ sections through C,D-D'' are marked by a broken line. Each image is a projection of 20 laser scanning fluorescent images (spanning a 30 µm total thickness) of GFP from a P{Ubi-GFP} transgene (green; B',D') and mouse anti-NICD primary antibody labelled with an anti-mouse Alexa-fluor^594nm secondary antibody (red; A-D) in control flies of genotype y w P{hsFLP}; P{neoFRT} P{M} Sb heph⁰³⁴²⁹/TM6B (A,C) or target class flies of genotype y w P{hsFLP}; P{neoFRT} P{M} Sb heph⁰³⁴²⁹/P{neoFRT} P{hsGFP} (B-B'',D-D'').

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