Fig. 5. The SPÄTZLE gene is required for endosperm cellularisation, but not for cytokinesis in the embryo. (A,B) The spätzle embryo does not show any morphogenetic defect (A, heart stage, B, torpedo stage) although it is surrounded by non cellularised MCE. (C) The PEN does not undergo cellularisation during the heart stage. However division of nuclei is maintained but is not always followed by proper separation of NCDs (D,E) and in some cases nuclei remain attached by incompletely separated nuclear envelopes (E, arrowhead). Hence multinucleated NCDs form and nuclei fuse (F) leading to large nuclei that display multiple nucleoli (G, arrowheads). Scale bars represent 20 µm.

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