Fig. 6. Anteroposterior polarity in cellularisation-defective mutants. (A,C,E) Cross sections of the posterior most part of the endosperm. In the wild-type the posterior-most part of the endosperm does not cellularise. (A) This part remains syncytial and contains two types of multinucleated large NCDs, the nodules (n) and the cyst (c). (C,E) In both knolle and spätzle backgrounds nodules and cysts are observed at the posterior pole similar to that in the wild type (early torpedo stage). In spätzle endosperm (E), the coalescence of NCDs is observed in the non-cellularised PEN and its distinction from the CZE is less clear than in the wild type or in knolle. (B,D,F) GFP expression in the enhancer trap line KS117. (B) After the globular stage, the posterior pole remains the only site where the GFP marker is expressed (Sørensen et al., 2001). (D,F) However, the expression of the marker KS117 is confined to the posterior pole as in the wild type in both knolle/+ (D) and spätzle/spätzle (F) backgrounds. Scale bar: 20 µm.

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