Fig. 2. Expression of cell adhesion markers in sal mutants. (A) Confocal optical sections show representative views of the expression of cell adhesion proteins in anterior nerve cord of embryos heterozygous (top row) or homozygous (bottom row) for the sal mutation at the stage when the strong adhesion phenotype was detected with TEM (early stage 16). (B) Fluorescence levels were consistently lower for Armadillo, N-Cadherin, Neuroglian, Fasciclin 2 and Fasciclin 3, but higher for Notch and normal for Neurotactin. Notice that Armadillo, N-Cadherin, Neurotactin and other markers are expressed by all CNS cells. The bars represent average levels for heterozygotes (black) and sal nulls (grey). Each bar shows the mean value for a minimum of five embryos processed, scanned and measured as detailed in Materials and Methods. Standard error is indicated. The most significant differences were found for Fasciclin 2 (P=0.001), Fasciclin 3 and N-Cadherin (P<0.005) and Armadillo (P<0.05). (C) When wild-type embryos were included in the comparison, the fluorescence levels for Armadillo, and N-Cadherin showed a gradual discrepancy from wild-type to null mutant embryos. The example shown here is Armadillo. Scale bar: 10 µm.

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