Fig. 7. sal mutant cell lineages (at stage 16) derived from single neuroectodermal precursors in mutant (A-E) or wild-type (F-I) background. Clones in A-E were obtained by labelling precursor cells (at stage 7) with DiI in situ, clones in F-I were obtained from HRP-labelled precursors upon transplantation (at stage 7) from mutant donors into wild-type hosts. All clones are located within abdominal neuromeres (A1-A4, shown as horizontal views, anterior towards the left). Drawings with light background (in A,B,D,F,G,I) show camera lucida tracings of the respective preparations. Drawings with dark background (in B-D,G-I) show identified wild-type lineages for comparison (see Bossing and Technau, 1994; Bossing et al., 1996; Schmidt et al., 1997). Glial cells are shown in green, neuronal cell bodies are in red and fibre projections are in black (see also arrows in B,C). In both series of experiments, there was a wide range of clonal phenotypes, including cases with no similarities to wild-type clones (A,F), cases with some components showing similarities to wild-type clones (B, NB1-3; G, NB4-2) and cases very similar to wild type clones (C, NB7-4; H, NB3-3). Along axons spherical thickenings were found (E, arrows). Although sal is not expressed in the CNS midline, some midline clones showed irregularities in their projection patterns (D, VUM clone; I, UMI clone).

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