Fig. 5. Expression of epidermal and neuroectodermal markers in HrzicN knockdown embryos examined before the tailbud stage. (A-C,E,K-O) Embryos injected with HrzicNMO. (F-H,J,P-T) Control uninjected embryos. (A,B,F,G,L,Q) Expression of HrETR-1, (C,E,H,J,M,R) Hroth, (K,P) HrEpiG, (N,S) HrTRP and (O,T) HrzicN. D and I are schematic representations of C and H, respectively; dots indicate blastomeres that exhibited Hroth expression. (A,C,F,H) Vegetal view of 110-cell stage embryos. (B,E,G,J) Animal view of 110-cell stage embryos. (K-T) Vegetal views. (K,P) Early gastrula equivalent stage embryos. (L-N,Q-S) Neural plate equivalent stage embryos. (O,T) Middle gastrula equivalent stage embryos. Arrowheads in B,E,G,J indicate gene expression in the sensory vesicle precursors. Arrows in C indicate ectopic expression of Hroth in the notochord precursors. Arrows in F show HrETR-1 expression in the A-line neural tube precursors, which is absent in HrzicN knockdown embryos (A). Brackets and braces in K and P indicate the region of the neural tube precursor cells of a-line and b-line, respectively, from which HrEpiG expression is excluded. Braces in L and O indicate A-line neural tube precursors without HrETR-1 expression and with HrzicN expression, respectively.

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