Fig. 3. Ebf1 is expressed in the dorsal thalamus and in the basal ganglia, along the path of thalamic axons. Coronal hemisections of E13.5 (A,A',B,B') or E14.5 (C,C') wild-type brains processed for Ebf1 in situ hybridization and Hoechst fluorescent cell stain. On the left, brightfield pictures showing Ebf1 expression are presented. On the right are fluorescent pictures of the same sections, where Ebf1 expression (open arrowheads in A',B',C') as well as low cell-density fiber tracts such as the thalamic axons (white arrowheads in A',B',C') appear in black. At E13.5, Ebf1 expression is observed in the mantle of the dorsal thalamus and of the ganglionic eminences (open arrowheads in A,B). Note that by E13.5, Ebf1 expression is undetected in the layer I of the neocortex. At E14.5, Ebf1 expression is observed in the amygdala and striatum (arrows in C) as well as in a group of cells located near the telencephalon-diencephalon boundary (open arrowhead in C). Ebf1-expressing cells are located on the path of thalamic axons at both ages (A',B',C'). dTH, dorsal thalamus; GE, ganglionic eminence; Ncx, neocortex.

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