Fig. 1. Bioinformatics and categorization of S. mediterranea sequences. (A) Organigram depicting the steps performed to produce a non-redundant collection of S. mediterranea cDNA sequences (SmedDb). Bioinformatic analyses compared the database against itself to identify and remove redundant clones before sending sequences to the public databases. The GenBank protein and nucleotide collections as well as the EST database (dbEST) were queried using the BLAST algorithm. Sequences returning significant matches (E10^-4) were subjected to annotation into functional categories based on the identity/function of the GenBank match. (B) Distribution of informative sequences by functional categories. For simplicity the cell signaling category includes the cell/cell communication and internal signaling categories. Metabolism is an amalgamation of the general metabolism, mitochondria and protein metabolism categories. Visit http://planaria.neuro.utah.edu for a detailed categorization profile and access to sequences. (C) Distribution by percentage of planarian genes displaying highest similarities with members of either the vertebrates or invertebrates.

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