Fig. 3. Labellar taste bristle and associated chemosensory neuron phenotypes of wild-type and Poxn null mutant males. (A-C) Labella with chemosensory taste bristles of w¹¹¹⁸ males (A), Poxn^M22-B5 males (B) and Poxn^M22-B5 males rescued with SuperA-207 (C) are compared by bright field microscopy at a resolution of 20x magnification. Arrows in B indicate bizarre shapes of shafts of transformed taste bristles, which are completely rescued to wild-type in C. (D-F) GFP expressing labellar chemosensory neurons of w; Poxn-Gal4-13-1 UAS-GFP/+ (D,F) and w; Poxn^M22-B5; Poxn-Gal4-13-1 UAS-GFP/BasiK109 (E) males are visualized by confocal fluorescence microscopy at resolutions of 20x (D,E) and 100x (F) magnification and with maximum projections of Z-stacks. Arrows in D,E indicate a group of GFP-expressing neurons innervating the labral sense organ (LSO) and in E a few remaining GFP-expressing neurons in the labellum. (F) Larger magnification of the labellum shown in D, illustrating the multiple innervation of the labellar taste bristles by chemosensory neurons: shaft of taste bristle (tb) is invaded by the dendrites of three chemosensory neurons (chs n).

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