Fig. 6. Turnover of tBR RNA within injected Xenopus embryos. Detection by ribonuclease protection assay of injected tBR mRNA in whole embryos. Embryos at the four-cell stage were radially injected with 250 pg tBR RNA per blastomere. Injected RNA was distinguished from endogenous BMP receptor RNA by probing for the Xenopus ß-globin UTR sequences present in the injected RNA. EF1 is the loading control. Note that at the end of gastrulation (stage 12) large amounts of tBR mRNA are detected but by neurula stages, the signal is all but gone. By early tail bud stage (stage 22), no tBR mRNA is detected at all.

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