Fig. 3. Removal of hh function partially suppresses the hyd mutant phenotype. All panels show third instar eye discs. (A-D) Anti-Elav staining in brown and X-gal staining, reflecting arm-lacZ expression (wild-type tissue), in blue. (A) Ectopic differentiation in hyd^K3.5 mutant clones. (B) hh^rJ413 clones; lack of photoreceptor differentiation is only apparent in clones at the posterior margin (arrow). (C) hyd^K3.5, hh^rJ413 double mutant clones. No ectopic photoreceptors form, but some clones still induce overgrowth of wild-type tissue (arrow). (D) hyd^K3.5, hh^rJ413 clones generated in a Minute background. The few photoreceptors that form are in wild-type tissue. (E) An adult eye containing hyd^K3.5, hh^rJ413 clones and exhibiting some overgrowth (arrow). (F) Unmarked hyd^K3.5 clones stained with anti-Elav in brown and X-gal, reflecting dpp-lacZ expression, in blue. dpp is ectopically expressed anterior to the furrow. (G,H) A third instar eye disc containing hyd^K3.5, hh^AC mutant clones (labeled by lack of GFP expression in H). Anti-ß-galactosidase (in red) is used to visualize dpp-lacZ expression; ectopic dpp is still visible in some clones (arrow in H).

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