Fig. 6. hyd affects hh expression and Ci accumulation in the wing disc. All panels show third instar wing discs with anterior to the left and dorsal up. (A,B) hyd^K7.19 mutant clones (marked by lack of GFP expression in B). hh-lacZ is shown in red and is increased in round posterior clones. (C-H) hyd^K3.5 mutant clones (marked by lack of GFP expression in D,F,H). Staining with anti-Smo is shown in red in C and D and staining with an antibody to the C-terminal region of Ci is shown in red in E,F. Anterior hyd mutant clones accumulate higher levels of full-length Ci, but do not accumulate Smo protein. (G,H) dpp-lacZ expression (in red). dpp expression is increased in clones near the AP boundary, but dpp is not activated in more anterior clones. (I-L) slmb^B93 mutant clones (marked by lack of GFP expression in J,L). Staining with an antibody to the C-terminal region of Ci is shown in red in I,J and is stronger than in hyd mutant clones. (K,L) dpp-lacZ expression (in red). dpp is misexpressed only in anterior clones that lie outside the wing pouch.

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