Fig. 2. shar-pei mutant clones in the eye show excess interommatidial cells, resistance to apoptosis and normal patterning. (A) Plastic thin section through an adult eye that is mosaic for shrp⁵ mutant cells. Mutant tissue lacks dark pigment granules in pigment and photoreceptor cells. Mutant ommatidial clusters have the normal complement of seven rhabdomeres in the correct trapezoidal arrangement. The spaces between the photoreceptor clusters, however, are significantly larger in mutant tissue than in wild-type tissue (arrowhead). (B,C) Mid pupal stage retinas with shrp⁴ mutant clones (B) and wild type (C) stained with anti-Dlg antibody to detect cell outlines. (D,E) Confocal section of the basal side of a 38 hours after puparium formation (APF) pupal retina mosaic for shrp³. Mutant cells are marked by the absence of GFP expression (shown in red). The retina was stained with antibodies against activated Drice to detect apoptotic cells (green in D). All apoptotic cells are wild-type and express GFP (arrowheads). (F) Cell outlines in a retina expressing p35 under GMR control revealed by Dlg expression. (G-L) shrp mutant clones in third instar eye discs marked by the absence of GFP (grayscale in G,J and blue in I,L). Discs are stained for Sens (green) and Elav (red) expression. (G-I) A shrp⁶ mutant clone spanning the morphogenetic furrow (arrowhead) that shows normal patterns of Sens and Elav expression. (J-L) A shrp¹ clone at the posterior edge shows normal patterning but increased spacing between ommatidial clusters (arrowhead). Anterior is towards the left in G-L.

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