Fig. 6. shar-pei mutant cells proliferate faster, but show normal cell cycle profiles and cell size. (A) Cell numbers in 50 shrp³ mutant clones (gray bars) and (B) 50 control clones of the isogenized wild-type FRT chromosome on which the shrp mutations were induced, compared with their twin clones (red bars). Twin clones were homozygous for an isogenized FRT 82B ubi-GFP^NLS chromosome. Cell numbers were counted in wandering third instar wing disc clones. (C) DNA profiles and (D) forward scatter distributions (FSC) of third instar wing disc cells measured by flow cytometry (FACS). shrp⁴ mutant clones were induced at 24-48 hours after egg laying (AEL) and wing discs dissected 72 hours after clone induction. Blue trace represents shrp mutant cells, red trace wild-type cells. Mutant and wild-type cells were sorted by GFP expression. The mutant cell population had a similar distribution of cell cycle profiles and cell sizes when compared with the wild-type cells. (E-G) shrp³ mutant clone in the presumptive wing pouch of a third instar wing disc marked by the absence of GFP expression (red). The disc is stained for Dlg to reveal cell outlines (green) and DNA to label nuclei (blue). Mutant cells have the same sized outlines as wild-type cells. Large cell outlines are from dividing cells showing apical mitotic figures (blue). (G) Merge of the three channels.

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