Fig. 7. Identification and sequence analysis of shar-pei. (A) Mapping of shrp relative to five P elements inserted in the 94A-96A region on 3R (horizontal line). Triangles show P elements with their names and genomic position in kb. Recombination distances between shrp (vertical line with star) and each P element are given in centiMorgan (cM, double arrows). (B) The genomic region of shrp determined by recombination mapping. Known and predicted ORFs are shown by arrowed boxes and genomic positions are given in kb above the DNA. The five gray boxed genes and shrp (black box) were sequenced. (C) Schematic representation of the protein structures of the fly, human and nematode Shrp homologs. Numbered arrows indicate the positions of the mutations in the six shrp alleles. The mutations in alleles 1-5 result in premature STOP codons, allele six has a +2 frameshift that results in the addition of 76 amino acids not related to any other protein in GenBank. (D) Sequence alignment of Drosophila Shrp (Dm), human WW45 (Hs) and C. elegans T10H10.3 (Ce). Identical residues are on black background, similar residues are shaded. The two WW domains are outlined by dark boxes and the shrp-specific domain is boxed by a broken line. Asterisks indicate the residues, the codons of which are mutated to STOP codons in the respective alleles, the position of the frame-shift in shrp⁶ is identified by an arrow. (E) Expression of shrp RNA in a wild-type eye-antennal disc. (F) The sense control shows no staining.

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