Fig. 5. Disp null mutants are defective in Hh signaling. (A-H,O-P) Whole-mount in situ hybridization using digoxigenin-labeled riboprobes on wild-type (A,C,E,G,O) and Disp^-/- (B,D,F,H,P) embryos at 9.5 dpc. All views are lateral. (A,B) Shh expression; (C,D) Ptch expression; (E,F) Hip1 expression; (G,H) Gli1 expression; (O,P) Disp expression. (I-N) Isotopic section in situ using ³³P-UTP-labeled riboprobes on wild-type (I,K,M) and Disp^-/- (J,L,N) embryos at 9.5 dpc. (J) Crosssection at the heart level. (I,K,L,M,N) Cross-sections at the forelimb level. (I,J) Shh expression; (K,L) Ptch expression; (M,N) Disp expression. nt, neural tube; fp, floor plate; nc, notochord.

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