Fig. 8. (A) Fgfr, Fgf and Bmp gene expression was localized to either epithelium or mesenchyme. E13 submandibular glands (n=20) were micro-dissected into epithelium (E) and mesenchyme (M) and the light micrographs show a typical gland before and after separation. Gene expression was analyzed by RT-PCR with GAPDH and S29 as controls. The experiment was repeated three times with similar results. (B) SU5402 treatment modulates gene expression of FGFRs, FGFs and BMPs. Expression of FGFs, FGFRs and BMPs was analyzed by real time PCR at 2, 6, and 20 hours of SU5402 treatment. E13 SMGs (n=6) were cultured with either 5 µM of SU5402 (black triangle) or an equal volume of DMSO (white square) for 2, 6 or 20 hours. Real time PCR was performed using SYBR Green PCR Master Mix and a TaqMan^TM 7700 thermocycler. The results are expressed as fold increase in gene expression compared to the control glands after 2 hours of culture. The reactions were run in triplicate, the experiment repeated three times, and the results were combined to generate the graphs.

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